DNA ligases seal five′-PO4 and 3′-OH polynucleotide finishes through a few nucleotidyl transfer techniques involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are crucial guardians of genomic integrity, and ligase dysfunction underlies human genetic condition syndromes. Crystal buildings of DNA ligases certain to nucleotide and nucleic acid substrates have illuminated how ligase response chemistry is catalyzed, how ligases acknowledge weakened DNA ends, And exactly how protein domain movements and Energetic-site remodeling are utilized to choreograph the tip-joining pathway.

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